Real-Time PCR and Sequencing Assays for Rapid Detection and Identification of Avian Schistosomes in Environmental Samples
نویسندگان
چکیده
منابع مشابه
Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
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ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
15 صفحه اولdetection and enumeration of cryptosporidium oocysts in environmental water samples by real-time pcr assay
introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were col...
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Real-time PCR assays were developed for the enumeration of plasmid-mediated quinolone resistance (PMQR) determinants, such as the qnrA, qnrB, and qnrS genes, in different water samples and chicken feces. The results indicate that the developed assays are specific and sensitive for the quantification of qnr genes in complex samples.
متن کاملRapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples
During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our convention...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2015
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00750-15